Facility

Proteomass Facility- Laboratory for Biological Mass Spectrometry “Isabel Moura”

Laboratory Director

Dr. Hugo Miguel Baptista Carreira dos Santos Ph.D. | MRSC

https://linktr.ee/HugoM.Santos

  • Assistant Researcher – FCT Investigator Program at LAQV-REQUIMTE-NOVA-FCT-Portugal
  • Adjunct Assistant Professor of Pathology-School of Medicine | University of Pittsburgh-USA
  • Editorial Advisory Board of Talanta – Elsevier

 Services

Proteomics & Bio–analytical Services 

  • We deliver scientific counselling in medical proteomics and proteomics.
  • We deliver scientific counselling in analytical biochemistry and medical biochemistry
  • Label-free protein quantification (Impact HD II Bruker)
  • 1D-GE SDS-PAGE
  • Off-gel IEF (Micro-rotofor Cell, BioRad)
  • Two-dimensional gel electrophoresis (2D-GE)
  • Gel imaging and spot picking (ProPic II)
  • In-gel digestion + MS and MS/MS (Bruker Ultraflex)
  • In-solution digestion + MS and MS/MS (Bruker Ultraflex)
  • MS and MS/MS (Bruker Ultraflex)
  • Purification and concentration of peptides (Millipore® ZipTips)
  • Reverse-phase Liquid Chromatography-MALDI-TOF/TOF (Accela Thermo + SunChrom MALDI Spotter + Bruker Ultraflex)
  • Liquid Chromatography DAD detection (Accela Thermo)
  • Speed vacuum for sample concentration/ evaporation
  • 96 well plate assays / ELISA / protein quantification (Labtech LT – 4000 MS and CLARIOstar BMG Labtech)

Bio–Molecular, and NANO–Material Spectroscopy

  • NanoDrop 1000 spectrophotometer
  • NanoDrop 3300 spectrofluorimeter
  • UV-vis studies
  • In-solution and solid-sate emission studies. Steady and Excited states
  • Nanoparticle synthesis and preparation
  • 96 well plate assays / cations / anions / aminoacid quantification ( CLARIOstar BMG Labtech)
  • DLS characterization (charge and size)

Prices

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