General Overview

II Hands-On Course dates: 23rd - 25th May 2013

Hands-On Course in Fluorescence and related techniques applied to Chemosensors

Maximum 12 persons. Course registration will be done by requesting order.

Attention: The course will be given in english and spanish.


An intensive laboratory and lecture course will be focus on the following themes:

1 - An introduction of the photophysical principles  applied to chemosensors

1.1 - Definition of the principal concepts of luminescence, fluorescence and phosphorescence.

Types of electronic transitions in polyatomic molecules

2 - Photophysical characterization: absorption, emission and excitation spectra

2.1 - Absorption spectra

2.1.1 - The Beer-Lambert Law

2.1.2 - The Franck-Condon Principle

2.2 - Emission and excitation spectra

2.2.1 - Radiative and non-radiative transitions

2.2.2 - Intermolecular and intramolecular de-excitation processes in fluorescence.

2.2.3 - Stokes shift

2.2.4 - Determination of Quantum Yields

2.2.5 - Effect of temperature

3 - An approach to a Colorimetric System: Spectrophotometric titrations with anions (F-, OH-, Cl- and Br-)

4 - Spectrophotometric and Spectrofluorimetric titrations with the transition metal ions Zn2+, Cu2+ and Hg2+

4.1 - Chelation enhancement of the fluorescence emission (CHEF)

4.2 - Chelation enhancement of the quenching emission (CHEQ)

4.3 - Heavy metal effect

4.4 - Pearson's theory

5 - Design of chemosensors

5.1. - A brief introduction of the different type of chemosensors. Definition of a fluorescent classical chemosensor

5.2 - Bio-inspired chemosensors

5.2.1 - Exploring photophysical parameters by NANOdrop instruments

6 - Application of MALDI-TOF-MS technique in the supramolecular chemistry

7 - Determination of thermodynamic constants interaction by spectrophotometric techniques

8.- Syntheis of naked metallic nanoparticules (Au and Ag) and modulation of the SPR band with sensorial purposes.


The overall aim of the course is to provide to each student with fundamental knowledge and hands-on experience necessary in the absorption and fluorescence field, in the design of new chemosensors, and learn very broad techniques improving his/her own research.