Hands On

  1. Pre-analytical considerations
    • Experimental design
    • Sample collection storage and preservation
    • Sample clean-up
    • Protein quantification

  2. Protein separation - 2D gel electrophoresis
    • IPG-IEF
    • SDS-PAGE

  3. In-gel trypsin digestion - accelerated method

  4. 18O-stable isotope labelling

  5. Principles of LC-MALDI
    • μHPLC Reverse phase peptide separation
    • Automated spotting

  6. MALDI-TOF/TOF-MS data acquisition

  7. Data analysis
    • Protein identification
    • Protein quantification