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Course Theory

The course is divided in two main modules, Module I - Theory and Module II - lab work

 

Module I - Theory

  • Fundamentals of proteomics
    • Protein fractionation: 1D-GE, 2D-GE, off-gel IEF
    • Sample preparation - In-gel trypsin digestion and 18o-isotopic labelling
    • Protein identification by Mass Spectrometry
    • Protein quantification by Mass Spectrometry

  • Experimental Design

  • Practical aspects of sample collection storage and preservation for the proteome analysis

  • Principles of LC-MALDI
    • μHPLC Reverse phase peptide separation
    • Automated spotting


Module II - Hands-on

  • Pre-analytical considerations
    • Experimental design
    • Sample collection storage and preservation
    • Sample clean-up
    • Protein quantification

  • Protein separation - 2D gel electrophoresis
    • IPG-IEF
    • SDS-PAGE

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  • In-gel trypsin digestion - accelerated method

  • 18O-stable isotope labelling

  • Principles of LC-MALDI
    • μHPLC Reverse phase peptide separation
    • Automated spotting

  • MALDI-TOF/TOF-MS data acquisition

  • Data analysis
    • Protein identification
    • Protein quantification

 

Attention: The course will be given in English and Spanish on language demand, thus, both will be used without any given restriction in this course .